3 BIGGEST tissue culture mistakes I made

1 TRANPLANTING TOO SOON Moving plants into their final substrate before they're truly ready can cause: growth stalls leaf loss root damage unnecessary stress WHAT I LEARNED: Wait until the plant is actively growing and showing strong root development before making any substrate changes. When in doubt, wait longer. 2 CONSTANTLY ADJUSTING THE SETUP It's tempting to keep checking, moving, and changing things-but tissue culture plants prefer stability. Examples: moving the plant around changing humidity levels switching substrates too early taking the dome on and off unnecessarily WHAT I LEARNED: Once I have a setup working, I leave it alone and let the plant do its job. Sometimes the best thing you can do is nothing. 3 BEING TOO GENTLE WHEN REMOVING AGAR I was so focused on protecting every root that I wasn't scrubbing aggressively enough to remove all of the agar. Leftover agar can cause: trapped moisture bacterial growth root rot WHAT I LEARNED: It's far better to lose a few fragile roots during cleaning than to leave agar behind. A few damaged roots can recover-rot usually doesn't. THE ACCLIMATION METHOD TM TISSUE CULTURE SURVIVAL GUIDE A step-by-step guide to successfully acclimating tissue culture plants at home. by Brianna